Restriction fragment length polymorphism analysis of Rhizobium galegae strains

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Restriction fragment length polymorphism analysis of Rhizobium galegae strains.

Total DNA of various Rhizobium galegae strains representing different geographical origins, and taxonomic divergence was digested with three restriction enzymes separately, Southern blotted, and hybridized with six heterologous probes. The sequence divergences for different pairwise comparisons were calculated from proportions of conserved hybridizing fragments. The unweighted pair group method...

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Restriction Fragment Length Polymorphism (RFLP)

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Applications of restriction fragment length polymorphism.

The availability of a variety of restriction endonuclease enzymes that cleave deoxyribonucleic acid (DNA) at specific sites has made it possible to identify the presence of polymorphic regions in the isolated fragments. Such restriction fragment length polymorphism (RFLP) results owing to a variation in the number of tandem repeats (VNTR) of a short DNA segment. These VNTR sequences can uniquel...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1989

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.171.10.5561-5566.1989